| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Karasinski, Gabriella N (9)
|
MAUN-8 |
|
40.49 |
- |
| 1 |
Hasis, Nicole (10)
|
MAWAY |
45.13 |
41.00 |
-4.13 |
| 2 |
Messmer, Gabriella B (10)
|
MAGSA |
|
42.05 |
- |
| 2 |
Stillwagon, Ella J (10)
|
AMGCY |
46.88 |
43.77 |
-3.11 |
| 3 |
Gounder, Kavya R (10)
|
MAGSA |
|
42.92 |
- |
| 3 |
Milhomme, Hadley (10)
|
MANLAC |
45.96 |
44.06 |
-1.90 |
| 4 |
Hickey, Lillian G (10)
|
MATRI |
49.89 |
43.13 |
-6.76 |
| 4 |
Turner, Hannah M (10)
|
MAEPAC |
48.43 |
49.22 |
0.79 |
| 5 |
Kifer, Hannah (10)
|
MASCAY |
|
44.06 |
- |
| 6 |
Smallwood, Charlotte R (9)
|
MATRI |
|
44.62 |
- |
| 7 |
Chen, Emily Y (10)
|
MASCAY |
|
47.62 |
- |
| 8 |
MacNeely, Camryn J (10)
|
MANLAC |
|
49.27 |
- |
| 9 |
Kelley, Peyton (10)
|
MAWASC |
|
50.98 |
- |
|
Torelli, Samantha C (9)
|
MATRI |
|
49.53 |
- |
|
Boughanmi, Tasneem M (9)
|
MAEPAC |
|
NS |
- |
|
Harris, Elise M (10)
|
MAEPAC |
|
NS |
- |